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    The specimen should be as large as possible and may include intact piecesof bone, shavings, scrapings, and/or excised necrotic material [28]. Wav-ing his arms and legs again, he soon makes another contact and the ballreacts again. Higher magnification ofthe callus from the area indicated bythe upperrectangle in panel a shows osteoblasts lining bone trabeculae.Most ofthe original fibrous and cartilaginous matrix atthis site has been replaced by bone

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    Numer-ous profiles ofrough-surfaced endoplasmic reticulum (rER)are present in the basal region.